fadA [2018-02-11T17:42:09.000Z]

acetyl-CoA C-acyltransferase

Locus: BSU32830

Isoelectric point: 5.18

Molecular weight: 40.96 kDa

Protein length: 391 aa

Gene length: 1173 bp

Function: fatty acid degradation

Product: acetyl-CoA C-acyltransferase

Essential: no

E.C.: 2.3.1.16

Synonyms: yusK

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids

Gene

Coordinates: 3,368,839 → 3,370,014

The protein

Catalyzed reaction/ biological activity

• Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA (according to Swiss-Prot)

Protein family

• thiolase family (according to Swiss-Prot)

Paralogous protein(s)

• MmgA, YhfS

Structure

• 3SS6 (the protein of B. anthracis, 41% identity, 73% similarity)

Expression and Regulation

Operons

• Genes: fadN-fadA-fadE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • SdpR: activation, PubMed, in SdpR regulon
  • FadR: repression, PubMed, in FadR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • FadN: induced by long-chain fatty acids (FadR) PubMed
  • expression of the operon is induced during biofilm formation PubMed

• Genes: fadM-fadN-fadA-fadE
  Description: PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • SdpR: activation, PubMed, in SdpR regulon

  Regulation

  • fadM: induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• MGNA-A603 (yusK::erm), available at the NBRP B. subtilis, Japan
• BKE32830 (ΔfadA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GACTGCCTCTTTCATAAGCT, downstream forward: _UP4_TTATGCTAAAGGGGGAAAAA
• BKK32830 (ΔfadA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GACTGCCTCTTTCATAAGCT, downstream forward: _UP4_TTATGCTAAAGGGGGAAAAA

References

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